activated pvdf membranes millipore Search Results


pvdf membrane  (Merck KGaA)
90
Merck KGaA pvdf membrane
Pvdf Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pvdf membrane - by Bioz Stars, 2026-06
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pvdf membranes  (Merck KGaA)
90
Merck KGaA pvdf membranes
Pvdf Membranes, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pvdf membranes - by Bioz Stars, 2026-06
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pvdf membrane  (Proteintech)
91
Proteintech pvdf membrane
Pvdf Membrane, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membrane - by Bioz Stars, 2026-06
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pvdf microporous membrane  (Merck KGaA)
90
Merck KGaA pvdf microporous membrane
Pvdf Microporous Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membranes  (Santa Cruz Biotechnology)
99
Santa Cruz Biotechnology pvdf membranes
Pvdf Membranes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membranes - by Bioz Stars, 2026-06
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immuno blot pvdf membranes  (Bio-Rad)
96
Bio-Rad immuno blot pvdf membranes
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immuno Blot Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immuno blot pvdf membranes - by Bioz Stars, 2026-06
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polyvinylidene fluoride membrane pvdf  (Merck KGaA)
90
Merck KGaA polyvinylidene fluoride membrane pvdf
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Polyvinylidene Fluoride Membrane Pvdf, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immobilon-p membrane  (Merck KGaA)
90
Merck KGaA immobilon-p membrane
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immobilon P Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
immobilon-p membrane - by Bioz Stars, 2026-06
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poly screen pvdf membranes  (DuPont de Nemours)
90
DuPont de Nemours poly screen pvdf membranes
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Poly Screen Pvdf Membranes, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poly screen pvdf membranes - by Bioz Stars, 2026-06
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polyvinylidene fluoride membrane  (Bio-Rad)
96
Bio-Rad polyvinylidene fluoride membrane
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Polyvinylidene Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinylidene fluoride membrane - by Bioz Stars, 2026-06
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supported pvdf membrane  (Merck KGaA)
90
Merck KGaA supported pvdf membrane
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Supported Pvdf Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supported pvdf membrane/product/Merck KGaA
Average 90 stars, based on 1 article reviews
supported pvdf membrane - by Bioz Stars, 2026-06
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0.45m polyvinylidene fluoride membrane  (Merck KGaA)
90
Merck KGaA 0.45m polyvinylidene fluoride membrane
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
0.45m Polyvinylidene Fluoride Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to PVDF membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).

Journal: Aging (Albany NY)

Article Title: Age-associated alterations in inducible gene transcription in human CD4 + T lymphocytes

doi:

Figure Lengend Snippet: Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to PVDF membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).

Article Snippet: Extracts were fractionated by electrophoresis through 10% SDS-poly-acrylamide gels, transferred to Immuno-Blot PVDF membranes (Bio-Rad) and probed with primary antibody [NF-κB p65 (C-20) rAB at 1:1000 (Santa Cruz), Sp1 (H-225) rAB at 1:1000 (Santa Cruz), TBP mAB at 1:500 (Abcam), beta actin mAb at 1:1000 (Abcam)].

Techniques: Purification, Incubation, Control, SDS Page, Binding Assay, Activation Assay